This research presents the primary detection of cardiac troponin I (cTnI), a significant biomarker for acute coronary syndrome prognosis (ACS), in human interstitial fluid (ISF) collected by way of electroporation. Measurements have been carried out utilizing molecularly imprinted polymer nanoparticles as artificial recognition components and a heat-transfer methodology inside a microfluidic system, yielding outcomes inside 15–20 min. This method demonstrated dependable cTnI quantification throughout a large, physiologically related focus vary of 0.1 to 1000 pg mL−1 in spiked ISF, reaching a superb detection restrict of 1.85 ± 0.32 pg mL−1. Comparisons with typical affected person pattern fluids have been carried out by repeating experiments with cTnI-spiked plasma and serum, which exhibited related detection limits of 1.78 ± 0.28 and 1.80 ± 0.22 pg mL−1, respectively. The developed sensor affords a fast, extremely delicate, non-invasive, and cost-effective platform for point-of-care ACS prognosis in ISF, doubtlessly bettering affected person outcomes and easing healthcare burdens.
