This research presents the primary detection of cardiac troponin I (cTnI), a significant biomarker for acute coronary syndrome analysis (ACS), in human interstitial fluid (ISF), which was collected utilizing electroporation. Measurements have been carried out utilizing molecularly imprinted polymer nanoparticles as artificial recognition parts and a heat-transfer technique inside a microfluidic system, yielding outcomes inside 15-20 min. This strategy demonstrated dependable cTnI quantification throughout a large, physiologically related focus vary of 0.1 to 1000 pg mL – 1 in spiked ISF, attaining a superb detection restrict of 0.41 ± 0.06 pg mL -1 . Comparisons with standard affected person pattern fluids have been performed by repeating experiments with cTnI-spiked plasma and serum, which exhibited related detection limits of 0.45 ± 0.01 and 0.49 ± 0.04 pg mL -1 , respectively. The developed sensor provides a fast, extremely delicate, non-invasive, and costeffective platform for point-of-care ACS analysis in ISF, probably bettering affected person outcomes and easing healthcare burdens.
