Growing environment friendly and environmental benign strategy for the remediation of antibiotic pollution has turn into paramount analysis crucial, because the intensive use of antibiotics has raised severe issues attributable to their potential to induce antibiotic resistance and disrupt ecological steadiness. On this work, we report the self-assembly of fluorenylmethyloxycarbonyl-lysine (Fmoc-Okay) aggregates with pure calf thymus DNA (CT-DNA) and Cu2+ to assemble catalyst that possesses copper-dependent energetic websites, mirroring the catalytic operate of laccase, an oxidase identified for its capacity to degrade phenolic antibiotics. Structural characterizations, together with round dichroism, fluorescence spectra, transmission electron microscopy (TEM) and electrons paramagnetic resonance (EPR), point out the affiliation of Fmoc-Okay to DNA parts, facilitating the coordination of Cu2+ to each. Kinetic research revealed that the Fmoc-Okay/CT-DNA/Cu²⁺ advanced exhibited over 13-fold greater catalytic effectivity than both CT-DNA/Cu²⁺ or Fmoc-Okay/Cu²⁺ alone. Notably, CT-DNA not solely serves as a structural scaffold but additionally promotes the entry of the antibiotic substrates (together with doxorubicin and tetracycline) to the copper heart attributable to its binding affinity for these antibiotics, thereby facilitating environment friendly oxidative degradation. This work affords a promising technique for developing high-performance, environmentally responsive metalloenzyme mimics for pollutant remediation.
