Graphical Summary
This work reported that P2 may self-assemble right into a hydrogel (gel 2) with a triple-helix construction, providing sustained broad-spectrum antimicrobial exercise and biocompatibility. In rat fashions, gel 2-coated titanium implants efficiently prevented S. aureus an infection and enhanced osseointegration. These outcomes spotlight gel 2 as a novel, antibiotic-free strategy for bettering outcomes in orthopedic surgical procedures.
Part snippets
Outcomes and dialogue
Penetratin is acknowledged for its average antimicrobial exercise. To reinforce its efficiency, we strategically mutated its non-conserved amino acids (Asparagine, Asn9 and Methionine, Met12) with hydrophobic amino acids (e.g., isoleucine, Ile, and Tryptophan, Trp), as these hydrophobic residues are identified to advertise membrane interplay and injury [28], [29] (Desk 1, Fig. S1–4). As proven in Desk 1 and Fig. S5, these mutations considerably enhanced the antimicrobial exercise of penetratin towards
Conclusion
On this examine, we engineered penetratin peptides by substituting non-conserved websites with hydrophobic amino acids, yielding candidates with enhanced antimicrobial properties. Amongst these, P2 uniquely self-assembled right into a hydrogel that includes a definite triple-helix construction. This hydrogel demonstrated broad-spectrum antimicrobial exercise, favorable biocompatibility, and a exceptional prolongation of efficacy, exceeding 48 instances that of its answer type. In comparison with typical β-sheet-based
Related content material
The Supporting Data is obtainable freed from cost
Authors
J.S. and L.W conceived the unique concepts and guided the mission. T.L., Q.Y., Y.C; L.W carried out the experiments. T.L. and Y.C analyzed all knowledge within the manuscript. J.S., T.L. and Q.Y wrote the manuscript. L.W.; D.Y, H.H. and B.Y edit the manuscript; W.T. affords amenities and tools together with micro-CT and X-ray irradiator. All authors learn and accepted of the ultimate manuscript.
T.L, Q.Y and Y.C contributed equally to this work.
CRediT authorship contribution assertion
Wenbin Liu: Writing – evaluate & enhancing, Conceptualization. HongKun Hu: Writing – evaluate & enhancing. Binwen Yuan: Writing – evaluate & enhancing. Weijun Tang: Sources. Dan Yuan: Funding acquisition. Tingting Li: Writing – authentic draft, Validation, Mission administration, Funding acquisition, Information curation. Yu Chen: Writing – authentic draft, Information curation. Qipeng Yan: Writing – authentic draft, Information curation. Junfeng Shi: Writing – evaluate & enhancing, Funding acquisition, Conceptualization.
Declaration of Competing Curiosity
The authors declare the next monetary pursuits/private relationships which can be thought of as potential competing pursuits: Junfeng Shi experiences monetary help was supplied by Nationwide Youth Expertise Assist Program. Dan Yuan experiences monetary help was supplied by the Nationwide Pure Science Basis of China. Tingting Li experiences monetary help was supplied by Pure Science Basis of Hunan province. Dan Yuan experiences monetary help was supplied by Pure Science
Acknowledgment
We recognize the technical help supplied by the Analytical Instrumentation Middle of Hunan College for Help in Confocal Microscopy. This work was partially supported by the Nationwide Pure Science Basis of China (32401127 to D.Y, 22505072 to T.L), Nationwide Youth Expertise Assist Program (202309460011), Pure Science Basis of Hunan province (2024JJ5072 to D.Y., 2025JJ80084 to T.L), The Key Mission of Hunan Provincial Training Division (22A0020 to D.Y.), Pure Science
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