Gene remedy, as a cutting-edge method for illness intervention, depends closely on developments in gene silencing methods. As an example, CRISPR-Cas9 has emerged as a number one gene-editing device attributable to its capacity to introduce exact cuts at particular genomic loci, enabling focused gene insertion, deletion, or modification. On this research, we developed a easy and efficient gene silencing technique by introducing a nucleic acid self-assembly module into the three’ untranslated area (UTR) of mRNA. This module demonstrated important gene silencing efficacy in eukaryotic cells by means of the formation of RNA aggregates. To systematically examine its regulatory mechanism on translation effectivity by means of the formation of higher-order RNA buildings, we quantitatively analyzed each mRNA and protein expression ranges. Moreover, our modular 3′ UTR sequences might be built-in with classical 5′ UTR parts (e.g., TOP sequences) to assemble a multidimensional post-transcriptional regulatory community. This know-how expands the variety of present UTR factor libraries and gives a reservoir of programmable regulatory parts for functions in artificial biology. It permits the development of orthogonal mixtures of multidimensional parts, tailor-made to particular gene expression regulation wants.
