Poly(tannic acid) (pTA) nanospheres, assembled from pure tannic acid molecules, exhibit robust intracellular antioxidant exercise and successfully modulate the invasive behaviour of triple-negative breast most cancers (TNBC) cells. Performing as redox-active nanostructures, pTA nanospheres suppress proliferation and induce mechanoregulatory adjustments, together with altered nuclear morphology, cytoskeletal disassembly, and diminished cell polarity. Particularly, pTA therapy causes spatial mislocalization of MT1-MMP from the invasive entrance to the perinuclear zone, disrupting its colocalization with F-actin and lowering its matrix-degrading capability. Excessive-resolution STED and TEM imaging reveal vimentin community collapse and mitochondrial redistribution alongside microtubules. Metabolic profiling reveals a marked decline in oxidative phosphorylation-linked ATP manufacturing. Regardless of these practical disruptions, cleaved caspase-3 stays undetectable, indicating a non-apoptotic, cytostatic state accompanied by autophagy and redox signalling compensation. These findings show that pTA nanospheres exert redox-driven mechanoregulation in TNBC cells, limiting their invasive potential with out inducing cell loss of life, and spotlight their promise as a non-lethal nanotherapeutics method for post-surgical or adjuvant management of metastatic development.
