Liu, Z. & Zhang, Z. Mapping cell varieties throughout human tissues. Science 376, 695–696 (2022).
Yamanaka, S. Pluripotent stem cell-based cell remedy—promise and challenges. Cell Stem Cell 27, 523–531 (2020).
Baysoy, A., Bai, Z., Satija, R. & Fan, R. The technological panorama and functions of single-cell multi-omics. Nat. Rev. Mol. Cell Biol. 24, 695–713 (2023).
Qiu, S. et al. Small molecule metabolites: discovery of biomarkers and therapeutic targets. Sign Transduct. Goal. Ther. 8, 132 (2023).
Rad, M. S., Cohen, L. B., Braubach, O. & Baker, B. J. Monitoring voltage fluctuations of intracellular membranes. Sci. Rep. 8, 6911 (2018).
Zhou, C., Zhao, W.-x., You, F.-t., Geng, Z.-x. & Peng, H.-s. Extremely secure and luminescent oxygen nanosensor primarily based on ruthenium-containing metallopolymer for real-time imaging of intracellular oxygenation. ACS Sens. 4, 984–991 (2019).
He, C., Lu, Ok. & Lin, W. Nanoscale metallic–natural frameworks for real-time intracellular pH sensing in reside cells. J. Am. Chem. Soc. 136, 12253–12256 (2014).
Zhang, X., Ogorevc, B. & Wang, J. Stable-state pH nanoelectrode primarily based on polyaniline skinny movie electrodeposited onto ion-beam etched carbon fiber. Anal. Chim. Acta 452, 1–10 (2002).
Soldà, A. et al. Glucose and lactate miniaturized biosensors for SECM-based high-spatial decision evaluation: a comparative examine. ACS Sens. 2, 1310–1318 (2017). This examine develops miniaturized enzymatic glucose and lactate biosensors utilizing Pt ultramicroelectrodes and enzyme immobilization to boost the sensitivity and spatial decision for monitoring metabolic exercise on the single-cell degree with SECM.
Yuan, X. et al. Versatile live-cell exercise evaluation platform for characterization of neuronal dynamics at single-cell and community degree. Nat. Commun. 11, 4854 (2020). This examine presents a dual-mode high-density microelectrode array platform that permits long-term, label-free electrophysiological imaging of neuronal cultures at subcellular, single-cell, and community ranges, integrating full-frame (19,584 electrodes) and high-SNR (246 channels) recording modes to help high-throughput evaluation of neuronal dynamics.
Jung, H. S. et al. CMOS electrochemical pH localizer-imager. Sci. Adv. 8, eabm6815 (2022).
Cools, J. et al. 3D microstructured carbon nanotube electrodes for trapping and recording electrogenic cells. Adv. Funct. Mater. 27, 1701083 (2017).
Zhou, X.-L., Yang, Y., Wang, S. & Liu, X.-W. Floor plasmon resonance microscopy: from single-molecule sensing to single-cell imaging. Angew. Chem. 132, 1792–1801 (2020).
Tanaka, H. et al. Potassium ion dynamics imaging by means of supported lipid bilayers with floor plasmon resonance microscopy. ACS Photonics 9, 3412–3420 (2022).
Shinohara, H., Sakai, Y. & Mir, T. A. Actual-time monitoring of intracellular sign transduction in PC12 cells by two-dimensional floor plasmon resonance imager. Anal. Biochem. 441, 185–189 (2013).
Nascimento, R. A. S. et al. Single cell “glucose nanosensor” verifies elevated glucose ranges in particular person most cancers cells. Nano Lett. 16, 1194–1200 (2016).
Li, X., Dunevall, J. & Ewing, A. G. Quantitative chemical measurements of vesicular transmitters with electrochemical cytometry. Acc. Chem. Res. 49, 2347–2354 (2016).
Hu, Ok., Vo, Ok. L. L., Hatamie, A. & Ewing, A. G. Quantifying intracellular single vesicular catecholamine focus with open carbon nanopipettes to unveil the impact of L-DOPA on vesicular construction. Angew. Chem. 134, e202113406 (2022).
Xie, C., Lin, Z., Hanson, L., Cui, Y. & Cui, B. Intracellular recording of motion potentials by nanopillar electroporation. Nat. Nanotechnol. 7, 185–190 (2012). This examine demonstrates using vertically aligned nanopillar electrodes to realize long-term, high-fidelity intracellular and extracellular recordings of cardiomyocyte motion potentials by forming tight membrane–electrode junctions and enabling reversible, localized electroporation to considerably cut back cell–electrode impedance, enabling the delicate pharmacological evaluation of ion channel exercise.
Jahed, Z. et al. Nanocrown electrodes for parallel and strong intracellular recording of cardiomyocytes. Nat. Commun. 13, 2253 (2022). This examine develops semi-hollow nanocrown electrodes for intracellular motion potential recordings, enablingparallel and long-term recording in a minimally invasive method.
Abbott, J. et al. A nanoelectrode array for acquiring intracellular recordings from hundreds of related neurons. Nat. Biomed. Eng. 4, 232–241 (2019). This examine presents a CMOS-based, massively parallel intracellular recording system integrating 4,096 vertical nanoelectrode websites, enabling the high-resolution measurement of chemical synapse traits and large-scale mapping of synaptic connectivity throughout neuronal networks.
Liu, R. et al. Extremely-sharp nanowire arrays natively permeate, report, and stimulate intracellular exercise in neuronal and cardiac networks. Adv. Funct. Mater. 32, 2108378 (2022).
Spira, M. E., Shmoel, N., Huang, S.-H. M. & Erez, H. Multisite attenuated intracellular recordings by extracellular multielectrode arrays, a perspective. Entrance. Neurosci. 12, 212 (2018).
Desbiolles, B. X. E., de Coulon, E., Bertsch, A., Rohr, S. & Renaud, P. Intracellular recording of cardiomyocyte motion potentials with nanopatterned volcano-shaped microelectrode arrays. Nano Lett. 19, 6173–6181 (2019).
Abbott, J. et al. CMOS nanoelectrode array for all-electrical intracellular electrophysiological imaging. Nat. Nanotechnol. 12, 460–466 (2017). This examine introduces a scalable 3D field-effect transistor array platform for the correct, minimally invasive recording of transmembrane potentials, enabling high-resolution measurements of intracellular sign conduction in cardiomyocytes and cardiac tissue constructs.
Gu, Y. et al. Three-dimensional transistor arrays for intra- and inter-cellular recording. Nat. Nanotechnol. 17, 292–300 (2022).
Hanif, S. et al. Natural cyanide embellished SERS lively nanopipettes for quantitative detection of hemeproteins and Fe3+ in single cells. Anal. Chem. 89, 2522–2530 (2017).
Lussier, F. et al. Dynamic-SERS optophysiology: a nanosensor for monitoring cell secretion occasions. Nano Lett. 16, 3866–3871 (2016).
Yan, R. et al. Nanowire-based single-cell endoscopy. Nat. Nanotechnol. 7, 191–196 (2012).
Rotenberg, M. Y. et al. Silicon nanowires for intracellular optical interrogation with subcellular decision. Nano Lett. 20, 1226–1232 (2020).
Shibata, T. et al. Photocatalytic nanofabrication and intracellular Raman imaging of dwelling cells with functionalized AFM probes. Micromachines 11, 495 (2020).
Ichikawa, T. et al. Protocol for reside imaging of intracellular nanoscale constructions utilizing atomic drive microscopy with nanoneedle probes. STAR Protoc. 4, 102468 (2023).
Penedo, M. et al. Visualizing intracellular nanostructures of dwelling cells by nanoendoscopy-AFM. Sci. Adv. 7, eabj4990 (2023).
Ding, H., Su, B. & Jiang, D. Latest advances in single cell evaluation by electrochemiluminescence. ChemistryOpen 12, e202200113 (2023).
Zhang, H. et al. Electrochemiluminescence-microscopy for microRNA imaging in single most cancers cell mixed with chemotherapy-photothermal remedy. Anal. Chem. 91, 12581–12586 (2019).
Zhou, X. et al. A brand new extremely selective fluorescent Ok+ sensor. J. Am. Chem. Soc. 133, 18530–18533 (2011).
Iamshanova, O., Mariot, P., Lehen’kyi, V. & Prevarskaya, N. Comparability of fluorescence probes for intracellular sodium imaging in prostate most cancers cell strains. Eur. Biophys. J. 45, 765–777 (2016).
Grienberger, C. & Konnerth, A. Imaging calcium in neurons. Neuron 73, 862–885 (2012).
Li, P. et al. A near-infrared-emitting fluorescent probe for monitoring mitochondrial pH. Chem. Commun. 50, 7184–7187 (2014).
Mita, M. et al. Inexperienced fluorescent protein-based glucose indicators report glucose dynamics in dwelling cells. Anal. Chem. 91, 4821–4830 (2019).
Tang, J. et al. Noninvasive and extremely selective monitoring of intracellular glucose through a two-step recognition-based nanokit. Anal. Chem. 89, 8319–8327 (2017).
Li, L. et al. Simultaneous quantitation of Na+ and Ok+ in single regular and most cancers cells utilizing a brand new near-infrared fluorescent probe. Anal. Chem. 87, 6057–6063 (2015).
Choe, M. & Titov, D. V. Genetically encoded instruments for measuring and manipulating metabolism. Nat. Chem. Biol. 18, 451–460 (2022).
Wu, J. et al. Movement-based DNA detection utilizing catalytic nanomotors. Nat. Commun. 1, 36 (2010).
Yu, X., Li, Y., Wu, J. & Ju, H. Motor-based autonomous microsensor for movement and counting immunoassay of most cancers biomarker. Anal. Chem. 86, 4501–4507 (2014).
Balasubramanian, S. et al. Micromachine-enabled seize and isolation of most cancers cells in advanced media. Angew. Chem. Int. Ed. 50, 4161–4164 (2011).
Esteban-Fernández de Ávila, B. et al. Single cell real-time miRNAs sensing primarily based on nanomotors. ACS Nano 9, 6756–6764 (2015).
Pal, M. et al. Helical nanobots as mechanical probes of intra- and extracellular environments. J. Phys. Condens. Matter 32, 224001 (2020).
Damage, R. C. et al. Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing micro organism. Nat. Biotechnol. 41, 919–931 (2023). This examine develops improved acoustic reporter genes that provide enhanced ultrasound distinction and secure in vivo expression for the non-invasive imaging of tumour colonization and gene expression.
Pleasure, B., Cai, Y., Bono, D. C. & Sarkar, D. Cell Rover—a miniaturized magnetostrictive antenna for wi-fi operation inside dwelling cells. Nat. Commun. 13, 5210 (2022). This examine introduces the Cell Rover, a magnetic antenna that’s able to wirelessly working inside dwelling cells, offering a platform for superior intracellular sensing.
Gómez-Martínez, R. et al. Silicon chips detect intracellular stress modifications in dwelling cells. Nat. Nanotechnol. 8, 517–521 (2013). This examine presents a silicon chip internalized into dwelling cells, enabling the direct measurement of intracellular stress modifications in a minimally invasive method.
Airaghi Leccardi, M. J. I. et al. Mild-induced rolling of azobenzene polymer skinny movies for wrapping subcellular neuronal constructions. Commun. Chem. 7, 249 (2024).
Rahmani, Ok. et al. Clever in-cell electrophysiology: reconstructing intracellular motion potentials utilizing a physics-informed deep studying mannequin educated on nanoelectrode array recordings. Nat. Commun. 16, 657 (2025). This examine develops a physics-informed deep studying mannequin to reconstruct intracellular motion potentials from extracellular recordings on nanoelectrode and microelectrode arrays, enabling high-throughput, non-invasive electrophysiology for cardiotoxicity assessments with out direct internalization of the probe for sensing.
Krumm, A. & Carey, C. Actual-time monitoring of mobile metabolic exercise: intracellular oxygen. Nat. Strategies 13, i–ii (2016).
Hu, Q. et al. Genetically encoded biosensors for evaluating NAD+/NADH ratio in cytosolic and mitochondrial compartments. Cell Rep. Strategies 1, 100116 (2021).
Giorgio, M., Trinei, M., Migliaccio, E. & Pelicci, P. G. Hydrogen peroxide: A metabolic by-product or a standard mediator of ageing alerts?. Nat. Rev. Mol. Cell Biol. 9, 722–728 (2007).
Shu, Y. et al. Remoted cobalt atoms on N-doped carbon as nanozymes for hydrogen peroxide and dopamine detection. ACS Appl. Nano Mater. 4, 7954–7962 (2021).
Jaworska, A., Malek, Ok. & Kudelski, A. Intracellular pH – benefits and pitfalls of surface-enhanced Raman scattering and fluorescence microscopy – a evaluation. Spectrochim. Acta A Mol. Biomol. Spectrosc. 251, 119410 (2021).
Yu, X.-M., Groveman, B. R., Fang, X.-Q. & Lin, S.-X. The function of intracellular sodium (Na+) within the regulation of calcium (Ca2+)-mediated signaling and toxicity. Well being (Irvine Calif.). 2, 8–15 (2010).
Shi, X.-M. et al. A supersmall single-cell nanosensor for intracellular Ok+ detection. CCS Chem. 3, 2359–2367 (2021).
Bootman, M. D. & Bultynck, G. Fundamentals of mobile calcium signaling: a primer. Chilly Spring Harb. Perspect. Biol. 12, a038802 (2020).
Shindo, Y., Yamanaka, R., Suzuki, Ok., Hotta, Ok. & Oka, Ok. Intracellular magnesium degree determines cell viability within the MPP+ mannequin of Parkinson’s illness. Biochim. Biophys. Acta Mol. Cell Res. 1853, 3182–3191 (2015).
Stauber, T. & Jentsch, T. J. Chloride in vesicular trafficking and performance. Annu. Rev. Physiol. 75, 453–477 (2013).
Bergwitz, C. & Jüppner, H. Phosphate sensing. Adv. Power Kidney Dis. 18, 132–144 (2011).
